Research on the Certifi cate accommodation scheme of “Industrial Robot Vision and Sensing Technology” under the “1+X” certifi cate system

In the exploration of “1+X” certificate system under the professional course of industrial robot technology certificate accommodation scheme, analyze the connotation of “1+X” certifi cate system, Aiming at the problems existing in the school, such as the mismatch between the professional courses of industrial robot technology and the vocational skill level certificate of “Industrial Robot Application Programming”, the low integration between the course content and the vocational skill level standard, and the lagging construction of professional teaching resource base, taking the course of “Industrial Robot Vision and Sensing Technology” as an example, According to the teaching practice, a professional course system matching the vocational skill level standard should be constructed, a diversifi ed and integrated teaching resource library should be created to realize the integration of courses and certifi cates, and the “1+X” certifi cate system should be integrated into the whole process of talent training

Panoramic Annular Localizer: Tackling the Variation Challenges of Outdoor Localization Using Panoramic Annular Images and Active Deep Descriptors

Visual localization is an attractive problem that estimates the camera localization from database images based on the query image. It is a crucial task for various applications, such as autonomous vehicles, assistive navigation and augmented reality. The challenging issues of the task lie in various appearance variations between query and database images, including illumination variations, dynamic object variations and viewpoint variations. In order to tackle those challenges, Panoramic Annular Localizer into which panoramic annular lens and robust deep image descriptors are incorporated is proposed in this paper. The panoramic annular images captured by the single camera are processed and fed into the NetVLAD network to form the active deep descriptor, and sequential matching is utilized to generate the localization result. The experiments carried on the public datasets and in the field illustrate the validation of the proposed system.Comment: Accepted by ITSC 201

Study on the vibration effect on operation subway induced by blasting of an adjacent cross tunnel and the reducing vibration techniques

The Hongshan road tunnels in Nanjing cross up the metro Line 1 tunnel, the closest distance between Hongshan road tunnels and subway tunnels is only 4.14 m. In order to ensure the safety of the subway structure during the Hongshan road tunnels group excavation blasting, the vibration of the subway tunnel was monitored real time. The monitoring results showed that the main frequency distributions of the radial, tangential and vertical vibration of subway tunnel were significantly different. The main frequency and energy of tunnel vibration is mainly concentrated in the high frequency band. This characteristic is very beneficial for the protection of the subway tunnel and catenary. A series of techniques to reduce the vibration were taken during tunnel excavation blasting, which reduced the impact of the blasting vibration to subway tunnel and catenary, and ensured the operation subway safety. The vibration of subway tunnel can be controlled within a certain safety standard with proposed of reducing vibration techniques. It is shown that real-time monitoring and the comprehensive application of the reducing vibration technique are able to guarantee the security of adjacent cross operation subway, which provides references for similar tunnel projects

Dissecting horizontal and vertical gene transfer of antibiotic resistance plasmid in bacterial community using microfluidics

The spread of antibiotic resistance genes (ARGs) has become an emerging threat to the global health. Although horizontal gene transfer (HGT) is regarded as one of the major pathways, more evidence has shown the significant involvement of vertical gene transfer (VGT). However, traditional cultivation-based methods cannot distinguish HGT and VGT, resulting in often contradictory conclusions. Here, single-cell microfluidics with time-lapse imaging has been successfully employed to dissect the contribution of plasmid-mediated HGT and VGT to ARG transmission in an environmental community. Using Escherichia coli with an ARG-coded plasmid pKJK5 with trimethoprim resistance as the donor, we quantified the effects of three representative antibiotics (trimethoprim, tetracycline and amoxicillin) on the ARG transfer process in an activated sludge bacterial community. It was found that HGT was influenced by the inhibitory mechanism of an antibiotic and its targets (donor, recipient alone or together), whereas VGT contributes significantly to the formation of transconjugants and consequently ARG spreading. Trimethoprim is highly resisted by the donor and transconjugants, and its presence significantly increased both the HGT and VGT rates. Although tetracycline and amoxicillin both inhibit the donor, they showed different effects on HGT rate as a result of different inhibitory mechanisms. Furthermore, we show the kinetics of HGT in a community can be described using an epidemic infection model, which in combination with quantitative measure of HGT and VGT on chip provides a promising tool to study and predict the dynamics of ARG spread in real-world communities

Integrating remote sensing, GIS and dynamic models for landscape-level simulation of forest insect disturbance

Cellular automata (CA) is a powerful tool for modeling the evolution of macroscopic scale phenomena as it couples time, space, and variables together while remaining in a simplified form. However, such application has remained challenging in forest insect epidemics due to the highly dynamic nature of insect behavior. Recent advances in temporal trajectory-based image analysis offer an alternative way to obtain high-frequency model calibration data. In this study, we propose an insect-CA modeling framework that integrates cellular automata, remote sensing, and Geographic Information System to understand the insect ecological processes, and tested it with measured data of mountain pine beetle (MPB) in the Rocky Mountains. The overall accuracy of the predicted MPB mortality pattern in the test years ranged from 88% to 94%, which illuminates its effectiveness in modeling forest insect dynamics. We further conducted sensitivity analysis to examine responses of model performance to various parameter settings. In our case, the ensemble random forest algorithm outperforms the traditional linear regression in constructing the suitability surface. Small neighborhood size is more effective in simulating the MPB movement behavior, indicating that short-distance is the dominating dispersal mode of MPB. The introduction of a stochastic perturbation component did not improve the model performance after testing a broad range of randomness degree, reflecting a relative compact dispersal pattern rather than isolated outbreaks. We conclude that CA with remote sensing observation is useful for landscape insect movement analyses;however, consideration of several key parameters is critical in the modeling process and should be more thoroughly investigated in future work

Study on the dynamic response of subway tunnel by viaduct collapsing vibration and the protective measures of reducing vibration

Collapsing vibration caused by demolition blasting of buildings has large effect on the buildings and underground structures nearby. Combined with the demolition blasting of a viaduct, numerical simulation was carried out to investigate dynamic response of subway tunnel subjected to collapsing impact load. The paper put forward composite protective structures of steel-rubber tires and makes safety checking calculation of the subway tunnel on the basis of composite protective measures by numerical simulation. In order to ensure the safety and stability of subway tunnel in the practical operation of demolition blasting of the viaduct, the composite protection system was further optimized, which may provide a good reference for the related engineering practices

Real-time study of rapid spread of antibiotic resistance plasmid in biofilm using microfluidics

Gene transfer in biofilms is known to play an important role in antibiotic resistance dissemination. However, the process remains poorly understood. In this study, microfluidics with time-lapse imaging was used for real-time monitoring of plasmid-mediated horizontal gene transfer (HGT) in biofilms. Pseudomonas putida KT2440 harboring an antibiotic resistance plasmid RP4 was chosen as the donor while Escherichia coli and activated sludge bacteria were used as the recipient cells. Dynamic features of the transfer process, including the transfer rate, cell growth rate and kinetic changes of the transfer frequency, were determined. It was found that the routes for gene transfer strongly depend on the structure and composition of a biofilm. While intraspecies HGT is essential to initiate a transfer event, the secondary retransfer from transconjugants to the same species is more efficient and can cause cascading gene spread in single-strain biofilms. For the activated sludge biofilm, only small and scattered colonies formed and vertical gene transfer appears to be the dominant route after initial intraspecies transfer. Furthermore, more than 46% of genera in the activated sludge were permissive to plasmid RP4, many of which are associated with human pathogens. These phenomena imply early prevention and interruptions to biofilm structure could provide an effect way to inhibit rapid antibiotic resistance gene spread and reduce the likelihood of catastrophic events associated with antibiotic resistance